Composite

Part:BBa_K1933201:Design

Designed by: Tomoki Uchino   Group: iGEM16_Kyoto   (2016-10-10)


constitutive expression of anti-Norovirus GII.4 scFv fused to BclA with 6xHis tag


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 531
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Silent mutation was made to remove PstI recognition site in BBaK1933002.

Source

  • BBa_K1933004: Promoter and RBS.
  • BBa_K1933000: The BclA surface expression component derives from Bacillus anthracis (34F2). It is a hair-like protein that covers B. anthracis’ spores.
    We outsourced chemical synthesis of this part for construction.
  • BBa_K1933002: scFv is made from fusing variable region (VH and VL chain) of a human anti-norovirus antibody with a linker peptide.
    We have been given the scFv-encoding plasmid (NoV GII.4 strain-specific antibody 12A2 (vector: pSCCA5-E8d)) from Dr. Kyoko Moriguchi of the Fujita Health University Department of Virology Parasitology.